Figure 2
From: Critical role of glycosylation in determining the length and structure of T cell epitopes
The probability of N- and O-glycosylation. Experimentally verified T cell epitopes (filled columns) (found in ImmuneEpitope Database) and randomly selected peptides with identical length from the same protein (gray columns) were analyzed for probable N- and O-glycosylation using Artificial Neural Network-based predictions. Probable glycosylation of ≥ 9 amino acid long peptides is shown in the figure. A peptide was considered to have probable glycosylation if the position of the predicted glycosylation site within the protein coincided with the position of the peptide. Figure 2A shows comparison of the numbers of predicted O-glycosylation sites for each experimentally verified T cell epitope and a randomly selected peptide with identical length from the same protein (Wilcoxon, p = 0.0019). Figure 2B shows comparison of predicted N-glycosylation of randomly selected, length-matched sequences and the functional T cell epitopes (Wilcoxon test, p = 0.00006).