Figure 3From: Automated processing of label-free Raman microscope images of macrophage cells with standardized regression for high-throughput analysisImage reconstruction (left) for specific SVD eigenvectors (right). A) Image reconstructed from the 1st eigenvector reveals a faint outline of the macrophage cell boundary and a non-flat field illumination in the microscope, as well as several dark spots within the cell. B) The 2nd eigenvector corresponds to one component of background noise. C) The reconstructed image using the 3rd eigenvector reveals a distinct macrophage cell boundary and nucleus (showing wavenumber region 2842.6 cm-1 to 3024.5 cm-1).Back to article page