Table 2 Summary of putative opossum cytokine genes including search strategy, best hit, predicted glycosylation sites and signal peptide information.
Identity | Search Strategy | Best Hit | Number of predicted glycosylation sites | Signal peptide identified | ||
|---|---|---|---|---|---|---|
Cytokines | Â | reference | e-value | N-gly | O-gly | opossum |
Interleukin 2 | synteny | - | - | 1 | 1 | Yes |
Interleukin 4 | synteny | - | - | 2 | 0 | Yes |
Interleukin 5 | BLAST | AAD37462.1 | 4e-028 | 1 | 0 | Yes |
Interleukin 6 | BLAST | NP_112445.1 | 0.081 | 2 | 3 | Yes |
Interleukin 10 | BLAST | AAD01799.1 | 3e-011 | 2 | 0 | Yes |
Interleukin 12A | BLAST | NP_032377.1 | 9e-006 | 1 | 1 | No |
Interleukin 13 | synteny/HMMER | - | - | 0 | 0 | No |
Interleukin 19 | BLAST | NP_001009940.1 | 3e-008 | 3 | 0 | Yes |
Interleukin 20 | BLAST | NP_061194.2 | 1e-004 | 0 | 0 | Yes |
Interleukin 21 | BLAST | Q9HBE4 | 0.088 | 2 | 1 | Yes |
Interleukin 22 | BLAST | NP_065386.1 | 3e-005 | 1 | 0 | Yes |
Interleukin 24 | BLAST | NP_006841.1 | 2e-006 | 2 | 0 | Yes |
Interleukin 26 | BLAST | NP_060872.1 | 3e-009 | 0 | 0 | No |
Interferon-γ | synteny | - | - | 2 | 0 | Yes |
Type 1 Interferons | Â | Â | Â | Â | Â | Â |
IFN-α (7 genes) | BLAST | AAO37656.1 | 1e-019 to 9e-974 (range) | 0–4 (range) | 0 | Yes |
IFN-β | BLAST | AAO37656.1 |  | 2 | 0 | Yes |
IFN-κ | BLAST | AAO37656.1 |  | 1 | 0 | No |
Cytokine receptors | Â | Â | Â | Â | Â | Â |
Common cytokine receptor gamma chain (IL-2Rγ) | BLAST | NP_000197.1 | 9e-019 | 6 | 3 | No |
Interferon-γ receptor 2 | BLAST | NP_005525.2 | 3e-018 | 3 | 2 | Yes |
T cell surface receptors | Â | Â | Â | Â | Â | Â |
CD4 | BLAST | NP_000607.1 | 5e-009 | 4 | 0 | Yes |
CD8 | BLAST | Q60965 | 9e-012 | 1 | 8 | Yes |