In silico characterization of immunogenic epitopes presented by HLA-Cw*0401
Immunome Research volume 3, Article number: 7 (2007)
HLA-C locus products are poorly understood in part due to their low expression at the cell surface. Recent data indicate that these molecules serve as major restriction elements for human immunodeficiency virus type 1 (HIV-1) cytotoxic T lymphocyte (CTL) epitopes. We report here a structure-based technique for the prediction of peptides binding to Cw*0401. The models were rigorously trained, tested and validated using experimentally verified Cw*0401 binding and non-binding peptides obtained from biochemical studies. A new scoring scheme facilitates the identification of immunological hot spots within antigens, based on the sum of predicted binding energies of the top four binders within a window of 30 amino acids.
High predictivity is achieved when tested on the training (r 2 = 0.88, s = 3.56 kJ/mol, q 2 = 0.84, s press = 5.18 kJ/mol) and test (AROC = 0.93) datasets. Characterization of the predicted Cw*0401 binding sequences indicate that amino acids at key anchor positions share common physico-chemical properties which correlate well with existing experimental studies.
The analysis of predicted Cw*0401-binding peptides showed that anchor residues may not be restrictive and the Cw*0401 binding pockets may possibly accommodate a wide variety of peptides with common physico-chemical properties. The potential Cw*0401-specific T-cell epitope repertoires for HIV-1 p24gag and gp160gag glycoproteins are well distributed throughout both glycoproteins, with thirteen and nine immunological hot spots for HIV-1 p24gag and gp160gag glycoproteins respectively. These findings provide new insights into HLA-C peptide selectivity, indicating that pre-selection of candidate HLA-C peptides may occur at the TAP level, prior to peptide loading in the endoplasmic reticulum.
Major histocompatibility complex (MHC) class I molecules, HLA-A, -B, and -C, are cell surface glycoproteins consisting of a polymorphic heavy α chain non-covalently linked to a light chain, β2-microglobulin (β2m). HLA-A and -B molecules play critical roles in cell mediated immune responses by binding short antigenic peptide fragments and presenting them on the surface of antigen-presenting cells for recognition by the CD8+ cytotoxic T lymphocyte (CTL). Although several HLA-C specificities with CTL epitopes have been reported [1, 2], much remains unknown with regards to their role in the immune response against viral antigens in part due to their poor expression at the cell surface [3, 4]. Recent research shows that this group of molecules plays a major role in the control of human immunodeficiency virus type 1 (HIV-1) infection . Improved understanding of peptide binding to this group of molecules is important in the study of HIV-1 disease progression, as well as the design of effective HIV peptide vaccines.
The HLA-C allele, Cw*0401, is of particular interest in the study of HIV-1 disease progression because it is the restriction element for HIV-1 proteins . Two HIV-1 proteins (p24gag and gp160gag) are currently known to be restricted by Cw*0401 . Cw*0401 is present in approximately 10% of the general population . The allele is expressed intracellularly in amounts comparable with HLA-A and -B molecules, but is poorly expressed at the cell surface [7, 8]. Improved understanding of peptide binding to this molecule is important for elucidating its role in HIV-1 disease progression.
Computational strategies for prediction of peptide binding to HLA-A and -B molecules are relatively advanced , while sequence-based predictive models for HLA-C molecules have encountered limited success due to the lack of experimental training data . Two matrix-based prediction algorithms for Cw*0401 were reported [11, 12], but a sequence independent approach is still lacking. To overcome these limitations, we have developed a structure-based predictive technique that integrates the strength of Monte Carlo simulations and homology modeling [13–15]. This method utilizes a probe or "base fragment" to sample different regions of the receptor binding site, followed by loop closure and refinement of the entire class I peptide. The technique has been successfully applied to analyze peptides binding to a variety of MHC class II alleles [14, 15]. In this work, we now extend our analysis to peptides presented by the class I HLA-C molecule. We investigated the HIV-1 p24gag and gp160gag peptide binding repertoire of Cw*0401 and illustrate that areas with high concentration of T-cell epitopes or "immunological hot spots" are potentially well distributed throughout both HIV-1 p24gag and gp160gag. We also show that Cw*0401 can possibly bind antigenic peptides in amounts comparable to both HLA-A and -B molecules. Characterization of predicted Cw*0401 binding sequences reveal that Cw*0401 may bind a large variety of amino acids at anchor positions with common physico-chemical properties which correlate well with existing experimental studies .
Results and discussion
Cw*0401 predictive model
High predictivity (r 2 = 0.88, s = 3.56 kJ/mol, q2 = 0.84, spress = 5.18 kJ/mol) is achieved when tested on the training dataset of 6 Cw*0401 peptide sequences. The Cw*0401 predictive model outperforms the predictive models done by Rognan et al.  on training datasets of 5 A*0204 (r 2 = 0.85, s press = 2.40 kJ/mol) and 37 2Kk (r 2 = 0.78, s press = 3.16 kJ/mol) peptide sequences and is comparable with our previous DRB1*0402 (r2 = 0.90, s = 1.20 kJ/mol, q2 = 0.82, spress = 1.61 kJ/mol) and DQB1*0503 (r2 = 0.95, s = 1.20 kJ/mol, q2 = 0.75, spress = 2.15 kJ/mol) prediction models on a training set of 8 peptides . The cross-validation coefficient q 2 and the standard error of prediction s press are stable, with q 2 = 0.84 and s press = 5.18 kJ/mol. This iterative regression procedure validates the internal consistency of the scoring function in the current model, rendering it suitable for predictions on the test dataset obtained from biochemical studies. The predictive performance of our model is further validated using the test dataset of 58 peptides. The external validation results indicate that our Cw*0401 predictive model is suitable for discriminating binding ligands from the background with high accuracy (AROC = 0.93) with sensitivity of 76% (SP = 0.95).
Characterization of Cw*0401 binding peptides
An in-depth analysis was performed to investigate the characteristics of Cw*0401 binding peptides. A panel of 2279 sequences was generated using an overlapping sliding window of size 9 across the entire p24gag  and gp160gag  glycoproteins and modeled into the binding groove of Cw*0401. From these sequences, a total of 877 binding sequences (predicted binders; SE = 76%, SP = 80%) were selected and a systematic analysis was performed to analyze the number of occurrence of individual amino acid residues and physico-chemical properties  at each position of Cw*0401 binding peptides.
Peptide position p2 is characterized by alanine (10%), glycine (13%), leucine (9%), serine (9%). 60% of the predicted residues at this position are hydrophobic in nature, while 93% are neutral. These properties correlate with the physico-chemical properties of existing binding motif (Tyr/Phe) at p2  as well as with the observed conservation in the test data (Table 1: Phe – 58% and Tyr – 26%). The p3 position shows a strong preference for glycine (11%) and threonine (8%). Existing anchor residues at this position are aspartic acid and histidine, which accounts for 9% of the total position-specific composition in the dataset. The p4 position shares similar characteristics as p3 (glycine: 9%; threonine: 8%), with additional preference for leucine (8%). Similar results were obtained at the p5 position (alanine: 8%; glycine: 9%; leucine: 8%). At p6, characteristic residues include glycine (9%) and leucine (8%). This position is favored by neutral (81%) acyclic (89%), medium/large (77%), and hydrophobic (51%) residues. The physico-chemical properties of these residues are in agreement with Val/Ile/Leu as reported in earlier studies  and is comparable with the conservation in the test dataset reported in Table 1 (Val – 29%, Ile – 12%, Leu and Pro – 10%). Finally, the p9 position was defined by six amino acids, including alanine (8%), glycine (10%), isoleucine (8%), leucine (8%), threonine (9%), and valine (8%). This position is primarily dominated by neutral (90%) and hydrophobic (58%) residues, and agrees with profiles of Leu/Phe as previously reported  and is consistent with the conservation in the test dataset reported in Table 1 (Leu and Phe – 39%,). Collectively, our data indicates that individual binding pockets may not be highly specific as previously reported  but can rather accommodate a wide-range of anchor residues with common physico-chemical properties. We attribute the discrepancies to two possibilities: i) the lack of extensive research on Cw*0401 peptides; and ii) natural peptides carrying these residues may be present in small amount and were thus not detected by experimental studies.
Prediction of p24gag and gp160gag immunological hot spots
The potential T-cell epitope repertoire (data not shown) and immunological hot spots (Figure 1) for HIV-1 p24gag and gp160gag are well distributed throughout both glycoproteins. The known p24gag epitope (p24 168–175) and gp160gag epitope (gp160 375–383) were successfully predicted by our model at the threshold of -200 (SE = 76%, SP = 80%) [5, 20]. At this threshold, the number of predicted hot spots for p24gag are fourteen (p24 20–75, 89–148, 143–262, 311–381, 447–506, 526–590, 664–727, 708–791, 873–930, 996–1053, 1054–1157, 1157–1316, 1308–1368, and 1378–1427), with an estimated FN = 3, and FP = 3 for SE = 0.76 and SP = 0.95 (Figure 1 and Table 2). For gp160gag we predict nine hot spots (gp160 44–89, 102–171, 174–289, 341–504, 489–559, 581–639, 667–721, 708–766, and 793–852), with an estimated FN = 2, FP = 2 for SE = 0.76 and SP = 0.95 (Figure 1 and Table 3). The results presented here indicate that Cw*0401 can bind antigenic peptides with specificities comparable to HLA-A and -B molecules, and any variability in antigen expression may be directly related to the loss or reduced cell surface expression of the molecule by mechanisms as yet unknown [8, 21].
Due to the low expression of HLA-C molecules at the cell surface, their role in cell mediated immune responses remain poorly understood. Collectively, the outcome of this analysis provides insights into the binding specificities of Cw*0401. Our data strongly indicate that Cw*0401 can bind antigenic peptides in amounts comparable to both HLA-A and -B molecules, and show the existence of a potentially large number of Cw*0401-specific T-cell epitopes that are evenly distributed throughout both HIV-1 p24gag and gp160gag glycoproteins. It remains to be determined what proportion of these peptides may be expressed at the cell surface and capable of eliciting functional responses. Probably, pre-selection of candidate HLA-C peptides may occur at the TAP level, prior to peptide loading in the ER . Consequently, a higher concentration of peptides is necessary for complexation with HLA-C molecules, resulting in their release from TAP. This provides a possible explanation for the reduced cell surface expression of HLA-C molecules .
The coordinates of Cw*0401 were obtained from the Protein Databank (PDB) with PDB code 1QQD . The structure was relaxed by conjugate gradient minimization, using the Internal Coordinate Mechanics (ICM) software .
Experimental binding data
The dataset comprises a total of 64 (57 binders and 7 non-binders) 9-mer peptides (Table 1). The available dataset is divided into training and testing datasets. Peptides with experimental IC50 values were selected as training data for optimizing the empirical free energy function (refer Empirical Free Energy Function). Due to the lack of experimental data, only 9 peptides with experimental IC50 values were identified, six (three binders and three non-binders) of which were used for training, while the remainder (four non-binders) were included in the test dataset as true negatives. Therefore, the training dataset contained six peptides with experimentally determined IC50 values (2 high-affinity binders, 1 medium-affinity binder, and 3 non-binders) derived from biochemical studies , while the testing dataset comprised the remainder 58 peptides (54 binders and 4 non-binders) [10, 18, 21, 24, 25]. Experimental IC50 values were classified as follows – high-affinity binders: IC50 ≤ 500 nM, medium-affinity binders: 500 nM < IC50 ≤ 1500 nM, low-affinity binders: 1500 < IC50 ≤ 5000 nM and non-binders: 5000 < IC50.
HIV-1 sequence data
The sequences of HIV-1 p24gag and gp160gag glycoproteins were obtained from UniProt . The accession numbers for p24gag and gp160gag glycoproteins used in this study are P04585 and P03377 respectively.
Docking was performed according to the procedure utilized in previous similar works [13–15]: (i) pseudo-Brownian rigid body docking of peptide fragments to the ends of the binding groove, (ii) central loop closure by satisfaction of spatial constraints, and (iii) refinement of the backbone and side-chain atoms of the ligand and receptor contact regions.
Empirical free energy function
The scoring function presented herein is based on the free energy potential in ICM . Computation of the binding free energy was performed according to previous similar work based on the difference between the energy of the solvated complex and the sum of the energy of the solvated receptor and that of the peptide ligand, followed by optimization using experimental IC50 values . This step was followed by 6-fold cross-validation for assessment of quality of the scoring function . In k-fold cross-validation, k random, (approximately) equal-sized, disjoint partitions of the sample data were constructed, and all given models were trained on (k-1) partitions and tested on the excluded partition. The results were averaged after k such experiments, and thus the observed error rate may be taken as an estimate of the error rate expected upon generalization to new data. The predictive power of the models was assessed by the cross-validation coefficient q 2 and the standard error of prediction s press .
Immunological hot spot prediction
In this study, 'immunological hot spots' are defined as antigenic regions of up to 30 amino acids and modeled according to previous similar work based on the sum of predicted binding energies of the top four binders within a window of 30 amino acids . Where available, these predicted hotspots were validated with available experimentally determined sites.
Training, testing and validation
The free energy scoring function was calibrated using 6 peptides with experimental IC50 values and tested on a dataset 58 peptides (54 binders and 4 non-binders) obtained from biochemical studies (Table 1). The predictive performance of our model was assessed using sensitivity (SE), specificity (SP) and receiver operating characteristic (ROC) analysis . SE = TP/(TP+FN) and SP = TN/(TN+FP), indicate percentages of correctly predicted binders and non-binders, respectively. TP (true positives) represents correctly predicted experimental binders and TN (true negatives) for experimental non-binders incorrectly predicted as binders. FN (false negatives) denotes experimental binders predicted as non-binders and FP (false positives) stands for experimental non-binders predicted as binders. The accuracy of our predictions was assessed by the ROC analysis where the ROC curve is generated by plotting SE as a function of (1-SP) for a complete range of classification thresholds. The area under the ROC curve (AROC) provides a measure of overall prediction accuracy, AROC < 70% for poor, AROC > 80% for good and AROC > 90% for excellent predictions .
cytotoxic T lymphocyte
human leukocyte antigen
major histocompatibility complex
human immunodeficiency virus
receiver operating characteristic
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This work was partly funded by the National Institute of Allergy and Infectious Diseases, National Institute of Health, USA (Grant #5 U19 AI56541 & Contract #HHSN266200400085C).
JCT carried out the computational modeling studies, participated in data analysis and drafted the manuscript. ZHZ helped in data collection and computational modeling studies. JTA, TWT, VB participated in experimental design. SR conceived the study, and participated in its design and coordination and finalized the manuscript. All authors read and approved the final manuscript.
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Tong, J.C., Zhang, Z.H., August, J.T. et al. In silico characterization of immunogenic epitopes presented by HLA-Cw*0401. Immunome Res 3, 7 (2007). https://doi.org/10.1186/1745-7580-3-7
- Anchor Residue
- Predicted Binding Energy
- Internal Coordinate Mechanic
- Previous Similar Work
- Peptide Binding Repertoire