Expression analysis of G Protein-Coupled Receptors in mouse macrophages

Immunome Research

Table 2 Macrophage-enriched GPCRs.

			Relative Normalised Expression
Receptor Class	Accession Number	Gene Name	BMM	TEPM	RAW264.7
Enriched in both BMM and TEPM
Class A
	NM_009779	C3ar1	1361.1	683.6	58.3
	NM_007577	C5r1	822.6	25.6	10.5
	NM_183168	P2ry6	434.0	94.0	196.4
	NM_009917	Ccr5	214.2	20.5	0.6
	NM_021476	Cysltr1	208.9	12.5	40.6
	NM_004230	Edg5	30.2	69.3	6.6
	NM_008967	Ptgir	24.7	72.1	1.7
	NM_009924	Cnr2	14.2	27.2	6.7
	NM_009912	Ccr1	14.1	235.1	16.3
	NM_145700	Ccrl1	11.1	26.6	12.7
	NM_008964	Ptger2	10.7	34.4	0.8
Class B
	NM_010130	Emr1	190	22.2	28.3

Micro-array analysis was used to identify GPCRs that were highly enriched in primary mouse macrophages (BMM and TEPM). GPCRs identified as highly enriched in BMM or TEPM were defined as GPCRs where the normalised value was as least 10-fold greater and significantly different (p-value < 0.05, Student's two-tailed t-test) from the median expression across all cell types. GPCRs are grouped according to their class type on the basis of sequence similarity as previously described [65–67].