Expression analysis of G Protein-Coupled Receptors in mouse macrophages

Immunome Research

Table 3 GPCRs induced by LPS in macrophages.

			Relative Normalised Expression
Receptor Class	Accession Number	Gene Name	BMM			TEPM
			2 h	6 h	24 h	1 h	7 h
Induced by LPS in BMM and TEPM
Class A
	NM_009630	Adora2a	1.2	28.2	3.3	4.2	52.2
	NM_007413	Adora2b	27.4	190.9	17.4	3.0	3.3
	NM_007719	Ccr7	1.0	5.1	1.9	0.4	2.9
	NM_017466	Ccrl2	39.2	65.7	46.7	13.8	9.2
	NM_010336	Edg2	7.3	5.7	0.3	1.0	10.0
	NM_007904	Ednrb	6.7	2.0	10.3	0.7	5.3
	NM_013521	Fpr1	5.9	120.9	23.3	1.0	21.4
	NM_008042	Fprl1	3.8	41.6	28.6	3.1	570.6
	NM_030701	Gpr109b	13.6	48.2	5.6	10.8	212.0
	NM_030720	Gpr84	8.7	10.3	0.2	7.8	47.5
	NM_145066	Gpr85	3.6	5.6	0.5	18.1	4.9
Class B
	NM_018782	Calcrl	1.0	2.6	0.2	0.6	6.9
Frizzled
	NM_021457	Fzd1	8.0	17.0	1.0	1.0	2.2

Micro-array analysis was used to assess the gene expression profile of GPCRs upon LPS treatment in primary mouse macrophages (BMM and TEPM). GPCRs regulated by LPS in primary mouse macrophages were defined as those that were 2-fold or greater up-regulated at 2, 6 or 24 h LPS in BMM and/or 1 or 7 h LPS in TEPM (and significantly different from controls, p-value < 0.05, Student's two-tailed t-test). Values shown depict gene expression relative to untreated control (0 h). GPCRs are grouped according to their class type on the basis of sequence similarity as previously described [65–67].