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Figure 5 | Immunome Research

Figure 5

From: Functional recombinant MHC class II molecules and high-throughput peptide-binding assays

Figure 5

Checkerboard titrations of DR4 α and β chains analyzed by ELISA. Various concentrations of urea denatured α and β chains were incubated with (A) and without (B) 2 μM HA306–318 in refolding buffer pH 8. After 48 h incubation, the assay was analyzed in the sandwich ELISA assay using streptaviding as capture reagent and L243 as detection antibody. (C) Signal to noise ratios were calculated from samples with and without HA306–318. In this case, the combination of 31 nM α chain and 1 nM β chain was determined to be optimal.

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