Figure 7
From: Functional recombinant MHC class II molecules and high-throughput peptide-binding assays
Checkerboard titrations of DR4 α and β chains analyzed by LOCI. Various concentrations of urea denatured α and β chains incubated with (A) and without (B) 2 μM HA306–318 in refolding buffer pH 8. After 48 h of incubation, the assay was analyzed by LOCI using L243 antibody coupled to acceptor beads and streptavidin coupled donor beads. (C) Signal to noise ratios were calculated from samples with and without HA306–318. In this case, concentrations of 6.3 nM α chains and 1.3 nM β chains were determined to be optimal.