Figure 8
From: Functional recombinant MHC class II molecules and high-throughput peptide-binding assays
Direct and competitive LOCI-based peptide-MHC-II binding assay. Titrations on a promiscuous malaria derived peptide YILLKKILSSRFNQM DR4: KD = 133 nM R2 = 0.98. DP401: KD = 0.8 nM R2 = 0.99. I-Ed: KD = 14 nM R2 = 0.98. DR2a: inhibition assay, reference peptide 4 nM Biotin-PEG-HA306–318 IC50 = 0.6 nM R2 = 0.98.