Figure 1

Average distances of glycosylation sites. The histogram shows frequency distribution of distances between glycosylation sites. It shows a markedly different pattern of N- and O-glycosylation. Most probable O-glycosylation sites are found less than 10 amino acids far from each other. In contrast, distances between probable N-glycosylation sites are highly variable. This indicates that O-glycosylation sites are found in clusters, while conditional N-glycosylation sites are more equally distributed throughout a protein sequence.