Critical role of glycosylation in determining the length and structure of T cell epitopes

Immunome Research

Table 1 Correlation of structural features of proteins with their autoantigenic character

Investigated parameter	P (MBM (Autoantigen, Xi)\|DN)	Kolmogorov-Smirnoff hypothesis test
Length	1	p < 0,001
EC N-glycosylation	1	p < 0,001
Total N-glycosylation	1	p < 0,001
Number of strand motifs	0.986774	p < 0,01
Number of transmembrane regions	1	p < 0,05
Number of turn motifs	0.005305	p < 0,05
Number of disulfide bridges	1	NS
Metal cofactor binding	0.097063	NS
Number of coiled coils	0.094628	NS
Number of alpha helices	0.014117	NS
Extracellular O-glycosylation	0.741702	NS
The presence of SigP	0.377562	NS
Intracellular N-glycosylation	0.132067	NS

Results of a Bayesian model-based analysis.
We calculated the posterior probability for each variable that it is directly dependent of the fixed variable, autoantigenicity of a protein (i.e., its probabilistic dependence is not mediated by other variables). This is formalized as being in the so called Markov Blanket Set of the variable Autoantigen, which is denoted with MBM (Autoantigen, Xi). Variables relevant to the autoantigen nature of proteins are listed in the table (posteriors below 0.001).