Figure 3
From: Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS
Global alterations of total and polysomal-bound mRNA in LPS-activated moDCs. The 54'675 probe sets present on the Affymetrix U133 Plus 2.0 GeneChip array were first filtered on expression (signal > 100 in all tested conditions) to obtain a preliminary list of 7'709 probe sets. The 7'709 probe sets were then filtered on fold change (applying a 2-fold cut-off) comparing Polysomal (Poly, red) and Total (Tot, blue) mRNA, between 0 h and 4 h (A and B) or 4 h and 16 h (C and D) post-LPS. For effective Venn diagram visualization, the transcriptionally up-regulated probe sets (A and C) were distinguished from the transcriptionally down-regulated probe sets (B and D). See Additional file 3 for a detailed description of the different genes subsets.