Table 2 Validation of the array data by real time PCR using total and polysome-bound RNA populations.
From: Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS
Gene Symbol | Affy probe set | RNA type | Fold change (p-value) Array | Fold change (+/- SD) qPCR | ||
|---|---|---|---|---|---|---|
4 h vs 0 h | 16 h vs 4 h | 4 h vs 0 h | 16 h vs 4 h | |||
IL-6 | 205207_at | Poly | 503,11 (0,040) | -7,3 (0,101) | 3641,21 (11,92) | -58,26 (1,21) |
Tot | 239,22 (0,010) | -7,3 (0,051) | 2017,22 (19,13) | -12,95 (2,11) | ||
RPL26 | 222229_x_at | Poly | 1,41 (0,205) | -1,78 (0,044) | 1,81 (0,32) | -1,06 (0,33) |
Tot | -1,36 (0,129) | -1,33 (0,321) | -3,12 (0,41) | -1,52 (0,34) | ||
RPL14 | 213588_x_at | Poly | 1,45 (0,008) | -1,47 (0,101) | 0,31 (0,12) | -0,81 (0,22) |
Tot | -1,1 (0,191) | -1,17 (0,089) | -1,31 (0,32) | -1,29 (0,33) | ||
RPS23 | 200926_at | Poly | 1,73 (0,099) | -2,74 (0,100) | 0,72 (0,13) | -2,52 (0,35) |
Tot | 1,05 (0,011) | -1,72 (0,111) | 0,32 (0,14) | -1,89 (0,07) | ||
CD80 | 1554519_at | Poly | 22,81 (0,133) | 1,31 (0,002) | 19,71 (0,72) | 0,77 (0,22) |
Tot | 33,65 (0,001) | 1,43 (0,005) | 22,71 (1,52) | 0,41 (0,11) | ||
OAS1 | 202869_at | Poly | 11,56 (0,048) | 1,36 (0,044) | 7,26 (1,24) | 1,74 (0,71) |
Tot | 13,12 (0,026) | 1,86 (0.003) | 12,32 (0,32) | 1,26 (0,11) | ||
OAS2 | 204972_at | Poly | 34,15 (0,043) | -1,16 (0,111) | 7,51 (0.12) | 1,48 (0,45) |
Tot | 23,5 (0,001) | 1,2 (0,011) | 5,91 (0,11) | 1,21 (0,22) | ||
CASP9 | 203984_s_at | Poly | -3,94 (0,011) | 2,92 (0,009) | -14,71 (1,3) | 2,79 (0,81) |
Tot | -3,74 (0,005) | 2,02 (0,001) | -11,7 (2,1) | 3,09 (0,62) | ||
HLA-F | 221875_x_at | Poly | 2,07 (0,021) | 1,21 (0,008) | 3,41 (0,42) | 1,97 (0,32) |
Tot | 1,92 (0,002) | 1,97 (0,007) | 2,31 (0,71) | 2,25 (0,55) | ||
INDO | 210029_at | Poly | 117,32 (0,022) | 1,34 (0,001) | 1176,21 (8,33) | 2,01 (0,26) |
Tot | 97,51 (0,011) | 1,91 (0,008) | 737,2 (3,55) | 2,54 (0,27) | ||
TAP1 | 202307_s_at | Poly | 6,62 (0,340) | 1,02 (0,009) | 4,71 (1,1) | 0,79 (0,20) |
Tot | 8,38 (0,013) | 1,31 (0,002) | 6,2 (0,89) | 1,55 (0,41) | ||
TAP2 | 225973_at | Poly | 3,19 (0,001) | 1,56 (0,111) | 3,01 (0,66) | 1,62 (0,61) |
Tot | 3,20 (0,013) | 1,95 (0,505) | 3,21 (0,52) | 1,93 (1,10) | ||
MD2 | 206584_at | Poly | -1,37 (0,008) | -3,43 (0,043) | -2,91 (0,77) | -3,30 (0,21) |
Tot | 1,11 (0,008) | -2,31 (0,002) | 1,51 (0,33) | -2,10 (0,41) | ||
eIF4B | 211938_at | Poly | -2,26 (0,001) | -1,65 (0,031) | -2,21 (0,23) | 0,87 (0,04) |
Tot | -3,81 (0,006) | -1,66 (0,003) | -17,91 (2,21) | 0,35 (0,02) | ||