Figure 2

Antibody binding affinity map. Heavy-chain amino acid sequences for germline and RV6-26 adult antibodies, and the RosettaDock CDR scoring profile (TFN). Regions likely to be at the interface (CDRs) are highlighted in blue for the germline sequence. Active mutations in RV6-26, which result in improved binding with VP6, are highlighted in red, while neutral or negative mutations are highlighted in green. The TFN sequence is the RosettaDock scoring profile defined for this study that rewards True (T, orange) interface residues in the CDR region known to make contact with antigens, penalizes False (F) interface residues not observed to make antigen contact, and makes no contribution for Neutral (N) interface residues that rarely make contact with antigens and non-CDR active residues.