Expression analysis of G Protein-Coupled Receptors in mouse macrophages

Immunome Research

Table 4 GPCRs repressed by LPS in macrophages.

			Relative Normalised Expression
Receptor Class	Accession Number	Gene Name	BMM			TEPM
			2 h	6 h	24 h	1 h	7 h
Repressed by LPS in BMM and TEPM
Class A
	NM_009924	Cnr2	0.2	0.1	0.3	0.3	0.1
	NM_009911	Cxcr4	0.3	0.1	7.1	0.7	0.1
	NM_183031	Ebi2	0.2	0.1	0.1	0.4	0.2
	NM_004230	Edg5	0.2	0.5	2.0	0.4	0.6
	NM_022320	Gpr35	0.2	0.4	1.6	0.6	0.1
	NM_030258	Gpr146	0.1	0.4	1.0	0.4	0.1
	NM_008772	P2ry1	0.3	0.1	0.2	0.3	0.1
	NM_175116	P2ry5	0.1	0.1	0.7	0.3	0.1
	NM_013641	Ptger1	0.7	0.5	0.3	0.7	0.3
	NM_008965	Ptger4	3.1	0.5	1.0	1.9	0.3

Micro-array analysis was used to assess the gene expression profiles of GPCRs upon LPS treatment in primary mouse macrophages (BMM and TEPM). GPCRs regulated by LPS in primary mouse macrophages were defined as those that were 2-fold or greater down-regulated at 2, 6 or 24 h LPS in BMM and/or 1 or 7 h LPS in TEPM (and significantly different from controls, p-value < 0.05, Student's two-tailed t-test). Values shown depict gene expression relative to untreated control (0 h). GPCRs are grouped according to their class type on the basis of sequence similarity as previously described [65–67].